"I ask the Almighty for the gift of a world no longer marked by antisemitism, nor by prejudice, oppression and persecution against any human being," the pope said.
International Holocaust Remembrance Day marks the anniversary of the liberation of Auschwitz-Birkenau in 1945.
The Holocaust resulted in the systematic murder of 6 million Jews and millions of others during World War II.
On Jan. 27, as he left his residence in Castel Gandolfo, Leo also spoke to reporters.
When asked about rising tensions in the Middle East and the deployment of a U.S. aircraft carrier to the region amid protests and killings in Iran at the hands of its regime, Leo emphasized the need "to pray hard for peace."
"We little ones can raise our voices and always seek dialogue rather than violence to resolve problems, especially on this day that commemorates the Shoah," he added.
In a post on X marking the Holocaust memorial, Pope Leo reaffirmed the Catholic Church’s commitment to the principles outlined in Nostra Aetate, the Second Vatican Council’s declaration rejecting all forms of antisemitism.
He stressed that the church "rejects any discrimination or harassment based on ethnicity, language, nationality or religion" and encouraged dialogue rather than violence as a means toward solving conflicts.
Fox News Digital has reached out to the Vatican for comment.